Biocompatibility Evaluation of Ag/Fe-TiO2 with AFSC

The biocompatibility of the obtained Ag/Fe-enhanced TiO₂ powders with AFSC was also evaluated based on fluorescence microscopy using RED CMTPX fluorophore (Thermo Fischer Scientific, Massachusetts, MA, USA), which is a cell tracker for long-term tracing of living cells. The CMTPX tracker was added in cell culture treated with the nanoparticles and the viability and morphology of the AFSC was evaluated after 5 days. The CMTPX fluorophore was added in the culture medium at a final concentration of 5 µM, incubated for 30 min in order to allow the dye penetration into the cells. Next, the AFSC were washed with PBS and visualized by fluorescent microscopy. The photomicrographs were taken with Olympus CKX 41 digital camera driven by CellSense Entry software (Olympus, Tokyo, Japan) [28 (link),31 (link)].