Polyvinyl alcohol (PVA), nylon 6, and chitosan (CS) polymer solutions were prepared as 4% (w/v), 8% (w/v), and 1% (w/v), respectively [38 (link)]. Three polymer mixes were combined and pushed into a spinneret using a 5 mL syringe with a 21 G/13 mm diameter, which was operated by an NE-300 pump (New Era Pump Systems, Farmingdale, NY, USA). The spinneret needle was charged with a voltage of 13.8 kV, using a high voltage power supply, Model FES-COS (Falco, New Taipei City, Taiwan). The needle tip and collector plate were 13 cm away. Then, the roller collector covered in baking paper was used to format electrospun PVA/nylon 6/CS nanofiber membranes. The membranes were observed using a thermal field emission scanning electronic microscope (FE-SEM, JEOL JSM-7800F, Akishima, Tokyo, Japan), and the diameter of the nanofiber membrane was measured by ImageJ software Version 1.53n (NIH, Bethesda, MD, USA) [38 (link)].
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