Generation and Culture of THP-1 Cell Lines

THP-1 cells were from the Cell Resource Center, IBMS, CAMS/PUMC (Beijing, China). The THP-1 cell line with Gsdmd knockout (THP-1^Gsdmd-KO) was generated previously [30 (link)]. Cell culture was performed at 37 °C in a 5% CO₂ humidified incubator. The culture medium was RPMI Medium 1640 basic (1×) (C22400500BT, Gibco) containing 10% (v/v) FBS (10099-141 C, Gibco), 1% penicillin and streptomycin (SV30010, HyClone). THP-1-Null, THP-1-defCasp1 (Casp1 knockdown), and THP-1-defNLRP3 (Nlrp3 knockdown) were purchased from InvivoGen and cultured as instructed by the manufacturer.

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Zhao Y, & Sun L. (2022). Bacillus cereus cytotoxin K triggers gasdermin D-dependent pyroptosis. Cell Death Discovery, 8, 305.

Publication 2022

RPMI Medium 1640 basic (1×) FBS THP-1-Null THP-1-defNLRP3

Cams Cell Cell culture Ibms Penicillin Streptomycin Thp 1 cell line

Corresponding Organization :

Other organizations : Institute of Oceanology

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Variable analysis

independent variables

Genetic modification of THP-1 cells (Gsdmd knockout, Casp1 knockdown, Nlrp3 knockdown)

dependent variables

Unspecified

control variables

Cell culture conditions (37 °C, 5% CO2, RPMI 1640 medium with 10% FBS and 1% penicillin/streptomycin)

controls

Positive control: THP-1-Null cells (wild-type)
Negative controls: THP-1-defCasp1 (Casp1 knockdown) and THP-1-defNLRP3 (Nlrp3 knockdown) cells

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