Isolation and Characterization of CEFFE

CEFFE was provided by SEME Cell Technology Co., Ltd. (Shanghai, China) and extracted as previously described [15 (link)]. Briefly, the sample was washed with saline several times, and then the clean fat layer was collected, mechanically emulsified and centrifuged to obtain a liquid layer. Finally, the liquid underwent a freeze–thaw process, followed by centrifugation to obtain the liquid layer, which was stored at − 80 °C. The protein concentration of CEFFE was measured by a BCA protein kit (Vazyme, China) and adjusted to 3 μg/μL.

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Liu M., Zhang D., Zhou X., Duan J., Hu Y., Zhang W., Liu Q., Xu B, & Zhang A. (2022). Cell-free fat extract improves ovarian function and fertility in mice with premature ovarian insufficiency. Stem Cell Research & Therapy, 13, 320.

Publication 2022

BCA protein kit

Cell Centrifugation Freeze Protein Protein a Saline

Corresponding Organization :

Other organizations : Ruijin Hospital, Shanghai Jiao Tong University

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Variable analysis

independent variables

Extraction method used to obtain CEFFE

dependent variables

Protein concentration of CEFFE

control variables

Washing the sample with saline several times
Mechanically emulsifying the clean fat layer
Centrifuging the emulsified sample to obtain a liquid layer
Subjecting the liquid layer to a freeze–thaw process
Centrifuging the liquid layer to obtain the final CEFFE sample
Storing the CEFFE sample at − 80 °C

controls

None specified

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