Metabolic Monitoring in Insulin-Deficient Mice

We measured glucose, body weight, and food intake every 5 days as previously described (1 (link), 6 (link), 8 (link)). We plotted survival to determine if LEPRs in RIP-Cre^25Mgn neurons are required or sufficient for leptin's capacity to reduce lethality in insulin-deficient mice. Free fatty acids (FFAs), ketone bodies, TG, and glycerol in blood were measured by commercially available kits (Wako diagnose, US; and Cayman US for glycerol) as described previously (1 (link)). Corticosterone (Cayman, US) and glucagon (#10-1271-01, Mercodia, US) in the plasma were measured by commercially available ELISA kits.