Immunoprecipitation and Western Blot Analysis

The antibodies used were also listed in Supplementary Table S2. As we described previously^{11 (link)}, cells were extracted for 30 min with immunoprecipitation lysis buffer (Beyotime). After centrifugation of the preparations, the concentrations of supernatants were measured with the BCA kit. Then 100 μg proteins were incubated with 14-3-3ε antibody at 4 ˚C overnight. Then the protein-antibody complex were incubated with IgA plus IgG sepharose beads (Beyotime) at 4 ˚C for another 12 h. After then, the supernatants were removed and the beads were washed for three times, resuspended in the SDS sample buffer (Beyotime), and boiled to remove protein from the beads. Then, such protein samples were analyzed by western blots.