ChIP was conducted according to the protocol in a previous study
[40] (link). Briefly, micrococcal nuclease was used to fragment chromatin. After that, the chromatin fragments were incubated with magnetic beads (Biogle, Wuxi, China). The DNA-bead complex was immunoprecipitated with anti-H3K27me3 antibody (Millipore, Billerica, USA) or negative control IgG. The primers for the promoter of the
RASGRP1 gene used for ChIP-qPCR were as follows: forward primer 5′-CTCTCCGAATTCCCCATTGTG-3′ and reverse primer 5′-AAATCAGAGCTGCATCCAC-3′.
[40] (link). Briefly, micrococcal nuclease was used to fragment chromatin. After that, the chromatin fragments were incubated with magnetic beads (Biogle, Wuxi, China). The DNA-bead complex was immunoprecipitated with anti-H3K27me3 antibody (Millipore, Billerica, USA) or negative control IgG. The primers for the promoter of the
RASGRP1 gene used for ChIP-qPCR were as follows: forward primer 5′-CTCTCCGAATTCCCCATTGTG-3′ and reverse primer 5′-AAATCAGAGCTGCATCCAC-3′.
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