Women who met study criteria were mailed instructions to follow an unrestricted diet for 3 days preceding the OGTT and to fast at least 10 hours the night before. At each in-person examination, fasting blood specimens were collected between 7:00 and 10:30 a.m. before consumption of 75 g of dextrose (Fisherbrand). Plasma aliquots obtained from fasting and 2-hour postload blood samples were stored at −70 °C until being transported to the University of Washington Northwest Lipid Metabolism and Diabetes Research Laboratories (Seattle, Washington), which performed enzymatic assays for glucose and double-antibody radioimmunoassays for total insulin developed by the Diabetes Endocrinology Research Center Immunoassay Core Laboratory (Seattle, Washington) (5 (link)).
Protocol detail
Oral Glucose Tolerance Test Procedure
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Antibody insulin
Blood
Diabetes
Diet
Endocrinology
Enzymatic assays
Glucose
Immunoassay
Lipid metabolism
Ogtt
Plasma
Radioimmunoassays
Women
Corresponding Organization :
Other organizations : Kaiser Permanente Oakland Medical Center, University of California, Davis
Top 5 similar protocols
Variable analysis
independent variables
- Unrestricted diet for 3 days preceding the OGTT
- Fasting blood glucose
- 2-hour postload blood glucose
- Fasting total insulin
- 2-hour postload total insulin
- Fasting for at least 10 hours the night before
- Blood specimens collected between 7:00 and 10:30 a.m.
- Consumption of 75 g of dextrose (Fisherbrand)
- Positive control: Not specified
- Negative control: Not specified
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