TBE and YF NTs were performed as described previously.
12 (link),
30 (link) Briefly, serially diluted serum samples were mixed with TBEV strain Neudoerfl
33 (link),
34 (link) or YFV strain 17D.
35 (link) BHK‐21 cells (ATCC) were added and incubated for 3 (TBEV) or 4 days (YFV). YF NT titers were expressed as the reciprocal of the serum dilution that prevented the development of a virus‐induced cytopathic effect (CPE). NT titers ≥ 20 were considered positive. Since TBEV strain Neudoerfl does not produce a CPE of BHK cells, inhibition of virus replication was determined by measuring the presence of the virus in the cell culture supernatants by ELISA.
36 (link),
37 (link) The NT titer was defined as the reciprocal of the serum dilution that gave a 90% reduction in the absorbance readout in the assay compared with the control without antibodies. NT titers ≥ 10 were considered positive.
12 (link),
30 (link) Briefly, serially diluted serum samples were mixed with TBEV strain Neudoerfl
33 (link),
34 (link) or YFV strain 17D.
35 (link) BHK‐21 cells (ATCC) were added and incubated for 3 (TBEV) or 4 days (YFV). YF NT titers were expressed as the reciprocal of the serum dilution that prevented the development of a virus‐induced cytopathic effect (CPE). NT titers ≥ 20 were considered positive. Since TBEV strain Neudoerfl does not produce a CPE of BHK cells, inhibition of virus replication was determined by measuring the presence of the virus in the cell culture supernatants by ELISA.
36 (link),
37 (link) The NT titer was defined as the reciprocal of the serum dilution that gave a 90% reduction in the absorbance readout in the assay compared with the control without antibodies. NT titers ≥ 10 were considered positive.
Free full text: Click here
