VDR Expression in Thyroid Carcinoma

The UALCAN database (http://ualcan.path.uab.edu/) was used to examine the expression levels of VDR according to different histology in thyroid carcinoma. The expression of VDR in PTC cell lines and tissues was further verified by Western blot analysis. Detailed procedures of Western blot analysis were performed as described previously [41 (link)]. The antibodies used were shown as follows: antibodies against VDR (D-6, sc-13133, Santa Cruz Biotechnology, 1:500), GAPDH (10494-1-AP, Proteintech, 1:3000), HRP-conjugated affinipure goat anti-mouse IgG(H+L) (SA00001-1, Proteintech, 1:5000), HRP-conjugated affinipure goat anti-rabbit IgG(H+L) (SA00001-2, Proteintech, 1:5000).