Screening Markers for Seedling Vigor

The markers located in the earlier identified genomic region associated with the traits improving seedling vigor of rice under DSR were screened. All the gene files for the reference genome were retrieved from RGAP. The selected markers then screened for the donor specificity using the merged variant file created using BCF tools. All the shortlisted markers were aligned with the reference genome using BLAST and the markers showing alignment at the multiple loci were rejected. Only the high specificity markers aligning at desired locus with low e-value were selected.

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Sandhu N., Singh J., Ankush A.P., Augustine G., Raigar O.P., Verma V.K., Pruthi G, & Kumar A. (2024). Development of Novel KASP Markers for Improved Germination in Deep-Sown Direct Seeded Rice. Rice, 17, 33.

Publication 2024

Corresponding Organization : Punjab Agricultural University

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Variable analysis

independent variables

Markers located in the earlier identified genomic region associated with the traits improving seedling vigor of rice under DSR

dependent variables

Seedling vigor of rice under DSR

control variables

Not explicitly mentioned

controls

Positive controls: Not mentioned
Negative controls: Not mentioned

Annotations

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