Data used in this study are summarized in Supplementary Table 1 online. The NRSF ChIP-chip data (GEO accession #: GSE8489) were obtained by analyzing the bound DNA fragments in Jurkat cells with Affymetrix Human Tiling 2.0R arrays. Two independent ChIP samples and two mock IPs were profiled. The NRSF ChIP-seq data were collected from a previous study4 (link). In that study, DNA fragments bound by NRSF in Jurkat cells were sequenced with the next generation sequencer made by Illumina/Solexa. These experiments involved sequencing a ChIP'd sample as well as a negative control sample generated from reverse-crosslinked genomic DNA that had not undergone immunoprecipitation. The Oct4 and Nanog ChIP-seq data were collected from [10].
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Transcription Factor Binding Profiles
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Corresponding Organization : Sequoia (United States)
Other organizations : Johns Hopkins University, HudsonAlpha Institute for Biotechnology
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Variable analysis
independent variables
- Chromatin immunoprecipitation (ChIP) of NRSF, Oct4, and Nanog
- Genomic DNA fragments bound by NRSF, Oct4, and Nanog in Jurkat cells
- Jurkat cells
- Reverse-crosslinked genomic DNA that had not undergone immunoprecipitation (negative control for NRSF ChIP-seq)
- Two independent ChIP samples for NRSF ChIP-chip
- Two mock IPs for NRSF ChIP-chip
- Reverse-crosslinked genomic DNA that had not undergone immunoprecipitation (negative control for NRSF ChIP-seq)
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