MSCs collected from swine subcutaneous abdominal fat tissue (5–10g) were digested in collagenase-H, filtered, and cultured for 3 weeks in advanced MEM medium (Gibco/Invitrogen) supplemented with 5% platelet lysate(Crespo-Diaz et al., 2011 (link)). Cellular phenotype was confirmed in both groups by cell-surface marker expression(Zhu et al., 2016 (link)), and the third passage preserved in Gibco Cell Culture Freezing Medium for subsequent studies.
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Isolation and Characterization of Swine MSCs
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Abdominal subcutaneous fat tissue
Cell
Cell culture
Collagenase
Culture medium
Phenotype
Platelet
Swine
Corresponding Organization : Mayo Clinic
Other organizations : First Affiliated Hospital of Jinan University, Mayo Clinic in Florida
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Variable analysis
independent variables
- Digestion of swine subcutaneous abdominal fat tissue (5–10g) in collagenase-H
- Filtering of the digested tissue
- Culturing the cells for 3 weeks in advanced MEM medium (Gibco/Invitrogen) supplemented with 5% platelet lysate
- Cellular phenotype confirmed by cell-surface marker expression
- Third passage cells preserved in Gibco Cell Culture Freezing Medium for subsequent studies
- No positive or negative controls were explicitly mentioned in the provided information.
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