PDA.Muc1KO and PDA.MUC1 as described by Besmer et al16 (link) are used. KCM and KCKO cell lines were generated from PDA.MUC1 and PDA.Muc1 KO mice, respectively.16 (link)Human PDA cell lines Hs766T, Capan-2, HPAFII, HPAC and CFPAC, BxPC3, Capan-1, and MIA-PaCa-2 were obtained from ATCC (Manassas, Va). BxPC3 cells were stably transfected with empty vector or vector containing full-length MUC1 to generate BxPC3.Neo and BxPC3.MUC1 cells, respectively. Dr Michael Hollingsworth generously donated mouse Panc02.Neo and Panc02.MUC1 cell lines.