NOD CD45.2 congenic (NOD.B6-Ptprcb/6908MrkJacJ) and NOD.SCID (NOD.CB17-PrkdcSCID/J) mice were purchased from Jackson Laboratories. NOD-Y3 IL-2Rβ+/+, NOD-Y3 IL-2Rβ +/−, NOD-Y3 IL-2Rβ−/− mice, where Y3 refers to the mutant IL-2Rβ transgene, were developed by backcrossing C57BL/6-Y3 IL-2Rβ−/− mice (32 (link)) to NOD/ShiLtJ mice for 12 generations. All Idd loci were confirmed to be of NOD origin. NOD/Foxp3-RFP reporter (FIR) mice were previously described (64 (link)) and crossed to NOD BDC2.5 and NOD-Y3 IL-2Rβ +/+ mice to yield NOD-Y3-FIR, NOD BDC2.5-FIR, and NOD-Y3 BDC2.5-FIR mice. All mice were housed in a specific pathogen-free animal facility at the University of Miami. The Institutional Animal Care and Use Committee at the University of Miami reviewed and approved all animal studies conducted in this study.
Protocol detail
NOD Mouse Strains for Immunology
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Animal
Institutional animal care and use committee
Mice
Mice il 6
Nod mice
Scid
Specific pathogen free
Transgene
Corresponding Organization : University of Miami
Other organizations : University of Colorado Denver
Top 5 similar protocols
Variable analysis
independent variables
- NOD CD45.2 congenic (NOD.B6-Ptprcb/6908MrkJacJ) mice
- NOD.SCID (NOD.CB17-PrkdcSCID/J) mice
- NOD-Y3 IL-2Rβ+/+ mice
- NOD-Y3 IL-2Rβ +/− mice
- NOD-Y3 IL-2Rβ−/− mice
- NOD/Foxp3-RFP reporter (FIR) mice
- NOD BDC2.5 mice
- NOD-Y3 BDC2.5-FIR mice
- Measured outcomes (not explicitly mentioned)
- All Idd loci were confirmed to be of NOD origin
- Positive control: NOD CD45.2 congenic (NOD.B6-Ptprcb/6908MrkJacJ) mice
- Negative control: NOD.SCID (NOD.CB17-PrkdcSCID/J) mice
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!