This procedure was based on previous studies [84 (link), 85 (link)]. Rats were habituated to experimenter handling and the open field arena. Rats were fasted 16 h before the experiment. On the day of the experiment, rats received a continuous IV infusion of vehicle (buffered saline), (S)-ketamine (10 mg/kg), or (R)-ketamine (10 mg/kg) over 40 min in an open field arena. Ten minutes after start of infusion, rats were injected (IP) with 13 MBq of 2-deoxy-2-[18F]fluoro-D-glucose (FDG; Cardinal Health). After 30 min, rats were anesthetized with 1.5% isoflurane, placed on a custom-made bed of a nanoScan small animal PET/CT scanner (Mediso Medical Imaging Systems) and scanned for 20 min on a static acquisition protocol, followed by a CT scan. The PET data were reconstructed and coregistered to an MRI template as described above. All statistical parametric mapping analyses were performed using Matlab R2016 (Mathworks) and SPM12 (University College London).
Protocol detail
PET Imaging of Ketamine Effects on Glucose Metabolism in Rats
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Corresponding Organization : Johns Hopkins University
Other organizations : National Institute of Neurological Disorders and Stroke, National Center for Advancing Translational Sciences, National Institute of Mental Health
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Variable analysis
independent variables
- (S)-ketamine (10 mg/kg)
- (R)-ketamine (10 mg/kg)
- Brain glucose metabolism measured by 2-deoxy-2-[18F]fluoro-D-glucose (FDG) PET
- Rats were habituated to experimenter handling and the open field arena
- Rats were fasted 16 h before the experiment
- Vehicle (buffered saline) infusion
- Positive control: (S)-ketamine (10 mg/kg)
- Negative control: Vehicle (buffered saline)
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