Gastric tumor cell lines HGC-27 and BGC-823, and human gastric mucosa epithelial cells GES-1 were purchased from Shanghai Cell Bank of Chinese Academy of Sciences (Shanghai, China). All tumor cells were cultured in Dulbecco's modified Eagle's medium (DMEM; Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) supplemented with 10% fetal bovine serum (FBS; Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA). GES-1 cells were cultured in Eagle's minimal essential medium (Sigma-Aldrich; Merck KGaA) supplemented with 10% fetal calf serum. All cells were cultured in a humidified atmosphere containing 5% CO2 at 37°C. Intracellular pH was analyzed as previously described (26 (link)).
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Intracellular pH Analysis in Gastric Cells
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Atmosphere
Cells
Chinese
Cultured medium
Epithelial cells
Fetal calf serum
Gastric
Gastric mucosa
Human
Intracellular
Tumor
Tumor cell lines
Corresponding Organization :
Other organizations : Tumor Hospital of Guangxi Medical University
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Variable analysis
independent variables
- Cell lines (HGC-27, BGC-823, and GES-1)
- Intracellular pH
- Culture conditions (humidified atmosphere, 5% CO2, 37°C)
- Cell culture media (DMEM for HGC-27 and BGC-823, EMEM for GES-1)
- Serum supplements (10% FBS for all cell lines)
- Positive control: Not explicitly mentioned.
- Negative control: Not explicitly mentioned.
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