In Vivo MPO Activity Detection

A luminol chemiluminescence assay was used to detect in vivo MPO activity (Gross et al., 2009 (link)). Mice were anaesthetized with 50 mg/kg pentobarbital sodium i.p. and injected i.p. with 150 mg/kg luminol sodium salt (Sigma-Aldrich) dissolved in PBS. Luminescence images were captured 10 min after the injection with an IVIS Lumina II imaging system (PerkinElmer) and processed using the Living Image software (PerkinElmer). Total radiance values (total photon flux/s) from standardized regions of interest of front and hind paws were used for quantitative analysis.

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Kovács M., Németh T., Jakus Z., Sitaru C., Simon E., Futosi K., Botz B., Helyes Z., Lowell C.A, & Mócsai A. (2014). The Src family kinases Hck, Fgr, and Lyn are critical for the generation of the in vivo inflammatory environment without a direct role in leukocyte recruitment. The Journal of Experimental Medicine, 211(10), 1993-2011.

Publication 2014

luminol sodium salt IVIS Lumina II imaging system Living Image software

Chemiluminescence assay Front regions Luminescence Luminol Mice Pentobarbital sodium Salt Sodium

Corresponding Organization :

Other organizations : Semmelweis University, Hungarian Academy of Sciences, University of Pecs, University of California, San Francisco

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Variable analysis

independent variables

Injection of 150 mg/kg luminol sodium salt (Sigma-Aldrich) dissolved in PBS

dependent variables

Total radiance values (total photon flux/s) from standardized regions of interest of front and hind paws

control variables

Mice were anaesthetized with 50 mg/kg pentobarbital sodium i.p.

controls

No positive or negative controls were explicitly mentioned.

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