Comprehensive Selenium Compounds Characterization

Chemical reagents. Sodium selenite and SeMet were purchased from Nacalai Tesque (Kyoto, Japan). Se-Methylseleno-l-cysteine (MeSeCys) was purchased from Acros Organics (Waltham, MA, USA). l-Selenocystine (SeCys2) and sodium selenate were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan) and Wako Pure Chemical Industries, Ltd. (Osaka, Japan), respectively. SeMetO was synthesized based on the method of Kubachka et al. [39 (link)]. 8-Anilino-1-naphthalenesulfonic acid (ANS) and 5,5′-dithiobis (2-nitrobenzoic acid) (DTNB) were purchased from Sigma-Aldrich (Shanghai, China). All chemicals were of reagent grade or higher. Ultrapure deionized water (DIW) with 18.3 MΩ cm resistance was used and was prepared using a Milli-Q system (Merck Millipore, Tokyo, Japan).