Histological Evaluation of Myocardial Samples

Experimental myocardial samples were fixed, embedded in paraffin, sectioned and mounted on glass slides. Hematoxylin-eosin stain (Sigma Aldrich, MI) was performed for histological analysis. Collagen deposition was detected using picrosirius red staining [20 (link)]. Luna’s technique was performed for the specific visualization of eosinophil granules [21 (link)]. The presence of eosinophils was also assessed by immunohistochemistry using mouse anti-eosinophil major basic protein (EMBP) antibody.

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Rios-Navarro C., Gavara J., Vidal V., Bonanad C., Racugno P., Bayes-Genis A., Miñana G., Husser O., Oltra R., Nuñez J., Chorro F.J., Bodi V, & Ruiz-Sauri A. (2018). Characterization and implications of the dynamics of eosinophils in blood and in the infarcted myocardium after coronary reperfusion. PLoS ONE, 13(10), e0206344.

Publication 2018

Hematoxylin-eosin stain

Anti antibody Collagen Embedded paraffin Eosin Eosinophil major basic protein Eosinophils Granules Hematoxylin stain Immunohistochemistry Mouse Myocardial

Corresponding Organization : St.-Johannes-Hospital Dortmund

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Variable analysis

independent variables

Experimental myocardial samples

dependent variables

Collagen deposition
Presence of eosinophils

control variables

Hematoxylin-eosin stain
Picrosirius red staining
Luna's technique
Immunohistochemistry using mouse anti-eosinophil major basic protein (EMBP) antibody

controls

Positive control: Not specified
Negative control: Not specified

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