Deep Brain Stimulation Protocol for Rats

DBS was performed with a digital stimulator (DS8000; World Precision Instruments, Sarasota, FL, USA) and stimulus isolator (DLS100; World Precision Instruments), using biphasic square pulses with 60 µs pulse width, 200 µs zero time and 130 Hz frequency. Stimulation intensities varied across experiments between 0 (=sham stimulation), 35, 75 and 100 µA. These stimulation parameters are comparable to previously reported work on DBS in rats (Guo et al., 2013 (link); Knapp et al., 2009 ; Levy et al., 2007 (link); Rouaud et al., 2010 (link); Tan et al., 2010 (link); Vassoler et al., 2008 (link)). Stimulation was always applied in the operant cage, started 5 min before actual testing and lasted for the duration of the entire session.

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Schippers M.C., Gaastra M., Mesman T., Schetters D., van Mourik Y., Denys D., Pattij T, & De Vries T.J. (2017). Deep brain stimulation of the nucleus accumbens core but not shell reduces motivational components of heroin taking and seeking in rats. Brain and Neuroscience Advances, 1, 2398212817711083.

Publication 2017

DS8000 DLS100

Pulses Rats

Corresponding Organization : Amsterdam Neuroscience

Other organizations : Academic Medical Center, University of Amsterdam

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Variable analysis

independent variables

Stimulation intensity (0 µA (=sham stimulation), 35 µA, 75 µA, 100 µA)

dependent variables

Unspecified

control variables

Digital stimulator (DS8000; World Precision Instruments, Sarasota, FL, USA)
Stimulus isolator (DLS100; World Precision Instruments)
Biphasic square pulses with 60 µs pulse width, 200 µs zero time and 130 Hz frequency
Stimulation applied in the operant cage, started 5 min before actual testing and lasted for the duration of the entire session

controls

Sham stimulation (0 µA) as a negative control

Annotations

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