Polymerase Chain Reaction Amplification

Polymerase chain reaction (PCR) was performed in a 20 µL reaction containing 8 ng of DNA, 0.2 µM of each primer, DreamTaq™ Hot Start PCR Master Mix (ThermoFisher Scientific Waltham, MA, USA) containing Taq polymerase, and all other reagents. PCR amplification was performed in a Primus 96+ thermocycler (MWG-Biotech^®, Luxembourg, Luxembourg), and amplicons were analyzed as described by Luro et al. (2008) with the ten pairs of primers used by Ferrer et al. (2021) [61 (link),62 (link)].

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Ferrer V., Paymal N., Costantino G., Paoli M., Quinton C., Tomi F, & Luro F. (2023). Correspondence between the Compositional and Aromatic Diversity of Leaf and Fruit Essential Oils and the Pomological Diversity of 43 Sweet Oranges (Citrus x aurantium var sinensis L.). Plants, 12(5), 990.

Publication 2023

DreamTaq™ Hot Start PCR Master Mix

Polymerase chain reaction Primers Taq polymerase

Corresponding Organization : Institut Agro Montpellier

Other organizations : Centre National de la Recherche Scientifique, Université de Corse Pascal Paoli

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Variable analysis

independent variables

Amount of DNA (8 ng)

dependent variables

Amplification of DNA fragments (analyzed as described by Luro et al. (2008) with the ten pairs of primers used by Ferrer et al. (2021))

control variables

Concentration of each primer (0.2 µM)
Use of DreamTaq™ Hot Start PCR Master Mix (ThermoFisher Scientific Waltham, MA, USA) containing Taq polymerase, and all other reagents
Thermocycler model (Primus 96+ thermocycler, MWG-Biotech®, Luxembourg, Luxembourg)

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