Immunohistochemical Analysis of Mismatch Repair Proteins

Immunohistochemical (IHC) analysis of MSH2, MSH6, PMS2, and MLH1 proteins was performed as previously described [16 (link), 19 (link), 20 (link)]. Briefly, after the tumor area adjacent to normal mucosa and/or lymphocytic infiltration had been marked, the paraffinized tissue was removed and multiple tissue blocks were prepared. Finally, 4 μm-thick sections were obtained for IHC following standard protocols. The mouse monoclonal antibodies used were anti-MSH2, anti-MSH6, anti-MLH1, and anti-PMS2 (BD Pharmingen, CA, USA).

Free full text: Click here

Qiu Y.Y., Zeng Y.X, & Cheng Y. (2023). Are High Levels of Microsatellite Instability and Microsatellite Stability Identical in DNA Mismatch Repair-Deficient Colorectal Cancer Patients?. Canadian Journal of Gastroenterology & Hepatology, 2023, 8370262.

Publication 2023

anti-MSH2 anti-MSH6 anti-MLH1 anti-PMS2

Lymphocytic Mlh1 Monoclonal antibodies Mouse Msh6 Mucosa Pms2 Tissue Tumor

Corresponding Organization : Chongqing Medical University

Other organizations : Chinese Academy of Medical Sciences & Peking Union Medical College

Top 5 similar protocols

Variable analysis

independent variables

IHC analysis of MSH2, MSH6, PMS2, and MLH1 proteins

dependent variables

Expression/presence of MSH2, MSH6, PMS2, and MLH1 proteins

control variables

Tumor area adjacent to normal mucosa and/or lymphocytic infiltration
Paraffin-embedded tissue sections
4 μm-thick tissue sections
Mouse monoclonal antibodies against MSH2, MSH6, MLH1, and PMS2

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!