Quantifying Immune Cell Infiltration in GC TME

We used the ssGSEA (single-sample gene-set enrichment analysis) algorithm to quantify the relative abundance of each cell infiltration in the GC TME. The gene set for marking each TME infiltration immune cell type was obtained from the study of Charoentong, which stored various human immune cell subtypes including activated CD8 T cell, activated dendritic cell, macrophage, natural killer T cell, regulatory T cell and so on (Table S2) [28 (link), 29 (link)]. The enrichment scores calculated by ssGSEA analysis were utilized to represent the relative abundance of each TME infiltrating cell in each sample.