Cell Culture and Viral Infection Protocols

HEK293T, Vero, and mouse embryonic fibroblasts (MEFs) were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Corning) supplemented with 10% heat-inactivated fetal bovine serum (FBS; HyClone), penicillin (100 U/ml), and streptomycin (100 μg/ml). Wild-type and Rig-i^−/− MEFs were prepared as described previously (42 (link)). Wild-type HSV-1 (KOS strain) and recombinant HSV-1 were generated and amplified in Vero cells as previously described (16 (link)), with viral titers ranging from 10⁷ to 10⁸ PFU/ml. Sendai virus was purchased from Charles River Laboratories.

Free full text: Click here

Huang H., Zhao J., Wang T.Y., Zhang S., Zhou Y., Rao Y., Qin C., Liu Y., Chen Y., Xia Z, & Feng P. (2021). Species-Specific Deamidation of RIG-I Reveals Collaborative Action between Viral and Cellular Deamidases in HSV-1 Lytic Replication. mBio, 12(2), e00115-21.

Publication 2021

DMEM Sendai virus

Cultured medium Eagle Embryonic Fibroblasts Hsv 1 Mouse Penicillin Rig i River Sendai virus Strain Streptomycin Vero cells

Corresponding Organization : University of Southern California

Top 5 similar protocols

Variable analysis

independent variables

Wild-type and Rig-i−/− MEFs

dependent variables

Not explicitly mentioned

control variables

HEK293T, Vero, and mouse embryonic fibroblasts (MEFs) cell lines
Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum (FBS), penicillin (100 U/ml), and streptomycin (100 μg/ml)
Wild-type HSV-1 (KOS strain) and recombinant HSV-1 with viral titers ranging from 107 to 108 PFU/ml
Sendai virus

controls

Positive control: Wild-type HSV-1 (KOS strain)
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!