Titers of plasma IgG and mucosal sIgA against the spike protein (S) of SARS-CoV-2 prototype strain and BA.1, BA.1.1, BA.2, BA.2.12.1, BA.2.75, and BA.4/5 were evaluated using the enzyme-linked immunosorbent assay (ELISA) as previously reported [13 (link)]. The optimal coating concentration of S protein of SARS-CoV-2 prototype strain and Omicron sublineages (ACROBiosystems, Beijing, China) were 50 ng. The optimal plasma dilutions were 1/400. The second antibodies were horseradish peroxidase conjugated goat anti-human Fc specific polyclonal IgG or goat rabbit anti-human α chain specific polyclonal IgA (Sigma Aldrich, St Louis, MO, USA), respectively.
Anti iggAntibodies Dilutions Enzyme linked immunosorbent assayGoatHorseradish peroxidase Human MucosalPlasma Rabbit Sars cov 2 Sars cov 2 s protein Siga Spike protein Strain
Other organizations :
Tianjin First Center Hospital, Tianjin Medical University, Nankai University, Sichuan University, West China Hospital of Sichuan University, Academy of Medical Sciences, MRC Human Immunology Unit, University of Oxford, Institute of Hematology & Blood Diseases Hospital
Titers of plasma IgG and mucosal sIgA against the spike protein (S) of SARS-CoV-2 prototype strain and BA.1, BA.1.1, BA.2, BA.2.12.1, BA.2.75, and BA.4/5
dependent variables
Coating concentration of S protein of SARS-CoV-2 prototype strain and Omicron sublineages
Plasma dilutions
control variables
Coating concentration of S protein of SARS-CoV-2 prototype strain and Omicron sublineages (50 ng)
Plasma dilutions (1/400)
Second antibodies (horseradish peroxidase conjugated goat anti-human Fc specific polyclonal IgG or goat rabbit anti-human α chain specific polyclonal IgA)
controls
No positive or negative controls were explicitly mentioned.
Annotations
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