The Sch-PAH mouse model was induced either by exposure to percutaneous infection using approximately 80 cercaria and animals used after 60 days of infection, or by murine intraperitoneal (IP) sensitization using 240 S. mansoni eggs/g body weight followed by intravenous (IV) injection of 175 eggs/g body weight after two weeks (21 (link), 22 (link)). The specific model is highlighted in each figure legend (i.e., cercariae or IP/IV Eggs). Tail vein injections were performed in 2.5% isoflurane-anesthetized mice. The depth of anesthesia was monitored based on the lack of response to toe pinch. The mouse tail was submerged in water at 37°C or warmed with a heating lamp held approximately 20 cm away, avoiding overheating or burning, for 20–30 s for dilation of veins following injection of the eggs diluted in 100 µl of phosphate buffer solution (PBS; 30G needle). Mice were monitored daily, and after 7 days, the animals were anesthetized using ketamine/xylazine (K/X at 100 and 10 mg/kg body weight; IP) for subsequent analysis. After the procedure, mice were euthanized via cervical dislocation. Alternatively, C57BL6 mice (Jackson Laboratory, Bar Harbor, ME) were nebulized with saline or Escherichia coli LPS (10 mg, 1 h daily for up to 4 days) (13 (link)). Strain- and age-matched mice were used as approved by the Institutional Animal Care and Use Committee.
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