FACS Screening of GPRC5D Phage Clones

Example 4

FACS Screening: FIG. 24 shows FACS analysis of GPRC5D-specific phage antibody clones (ET150-1, ET150-2, ET150-5, ET150-8, ET150-18). Phage clones were incubated with 3T3-GPRC5D cell line, then with anti-M13 mouse antibody. Finally APC-labeled anti-mouse IgG 2nd antibody was added to the reaction after washing again. The binding was measured by FACS and expressed as mean fluorescence intensity (MFI). Cells incubated with M13 K07 helper phage and cells only were used as negative controls.

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US11866478B2. Nucleic acid molecules encoding chimeric antigen receptors targeting G-protein coupled receptor (2024-01-09). MEMORIAL SLOAN KETTERING CANCER CENTER [US], EUREKA THERAPEUTICS, INC. [US]. Inventors: Renier J. Brentjens [US], Eric L Smith [US], Cheng Liu [US].

Patent 2024

3t3 cell Anti antibodies Anti antibody Antibody Cell line Cells Clones Fluorescence Gprc5d Igg antibody Mouse Phage Phage m13

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Variable analysis

independent variables

GPRC5D-specific phage antibody clones (ET150-1, ET150-2, ET150-5, ET150-8, ET150-18)

dependent variables

Binding as measured by FACS and expressed as mean fluorescence intensity (MFI)

control variables

M13 K07 helper phage
Cells only

Annotations

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