JZL184, an inhibitor for MAGL (Cayman Chemical), was administered to C57BL/6 N mice, as described previously with minor modifications16 (link). To test its effects on the PGE2 contents, JZL184 dissolved in 18:1:1 mixture of saline, ethanol and Cremophor (Nacalai) or vehicle solution without JZL184 was injected intraperitoneally (i.p.) at 40 mg/kg at least 60 min before single exposure to SDS. To test its effects on repeated SDS-induced behavioral changes, JZL184 was administrated 6 h before each exposure to SDS.
SC-560 (Merck) and SC-236 (Cayman Chemical) that are inhibitors for COX1 and COX2, respectively, were administered to C57BL/6N mice, as described previously with minor modifications13 (link). SC-560 and SC-236 dissolved in DMSO or vehicle solution alone were injected i.p. at 5 mg/kg at 30 min before the last exposure to SDS (i.e. 10th SDS).
SC-560 (Merck) and SC-236 (Cayman Chemical) that are inhibitors for COX1 and COX2, respectively, were administered to C57BL/6N mice, as described previously with minor modifications13 (link). SC-560 and SC-236 dissolved in DMSO or vehicle solution alone were injected i.p. at 5 mg/kg at 30 min before the last exposure to SDS (i.e. 10th SDS).
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