EdU incorporation was used to assess the in vivo cell proliferation following instruction from the manufacture (#C10337, Thermo Fisher Scientific). Cell quantification was performed using Fiji imaging software (1.52p) as described previously 5 (link). Apoptosis was evaluated by TUNEL assay (#S7110, MilliporeSigma). E15.5 lung specimens with additional treatment of DNase I served as the positive control.
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In vivo Cell Proliferation and Apoptosis
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Corresponding Organization : University of Cincinnati Medical Center
Other organizations : McMaster University, University of Pennsylvania, University of Michigan–Ann Arbor
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Variable analysis
independent variables
- EdU incorporation
- Cell proliferation
- Apoptosis
- Cell quantification using Fiji imaging software (1.52p)
- TUNEL assay (#S7110, MilliporeSigma)
- E15.5 lung specimens with additional treatment of DNase I as positive control
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