Hematoxylin and Eosin (H&E) staining was done according to the previously described method [17 (link),18 ]. The slides were incubated with Harris hematoxylin for 10 seconds, washed, treated with eosin (Sigma Aldrich Co., St. Louis, MO, USA) for 5 seconds, and washed again. After drying, the slides were dehydrated by soaking in ethanol and xylene, and sealed with Permount (Thermo Fisher Scientific). Images were captured using an Image Analysis System (Leica Microsystems, Wetzlar, Germany).
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Hematoxylin and Eosin Staining Protocol
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Corresponding Organization :
Other organizations : Kangnam University, Kyung Hee University
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Variable analysis
independent variables
- Incubation time with Harris hematoxylin (10 seconds)
- Incubation time with eosin (5 seconds)
- Staining intensity/pattern
- Staining protocol (previously described method [17,18])
- Staining reagents (Harris hematoxylin, eosin from Sigma Aldrich Co.)
- Dehydration and mounting (ethanol, xylene, Permount)
- Imaging system (Leica Microsystems)
- Positive control: Not mentioned
- Negative control: Not mentioned
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