Abdominal Aorta Endothelial Protein Expression

The abdominal aorta was isolated, fixed in 4% PFA for 12 min, opened in the longitudinal direction, and stained for ICAM1 (Abcam), VCAM1 (Abcam), or VE-cadherin (Abcam) in combination with DAPI. En face sections were obtained on a Leica SP8 confocal microscope, and all the analyses were performed with Image J [15 (link)].

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Poels K., van Leent M.M., Reiche M.E., Kusters P.J., Huveneers S., de Winther M.P., Mulder W.J., Lutgens E, & Seijkens T.T. (2020). Antibody-Mediated Inhibition of CTLA4 Aggravates Atherosclerotic Plaque Inflammation and Progression in Hyperlipidemic Mice. Cells, 9(9), 1987.

Publication 2020

ICAM1 VCAM1 VE-cadherin SP8 confocal microscope

Abdominal aorta Confocal microscope Dapi Face Icam1 Ve cadherin

Corresponding Organization : Vrije Universiteit Amsterdam

Other organizations : Icahn School of Medicine at Mount Sinai, Eindhoven University of Technology, Ludwig-Maximilians-Universität München, German Centre for Cardiovascular Research

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Variable analysis

independent variables

Staining for ICAM1 (Abcam), VCAM1 (Abcam), or VE-cadherin (Abcam)

dependent variables

En face sections obtained on a Leica SP8 confocal microscope
Analyses performed with ImageJ

control variables

Abdominal aorta isolated and fixed in 4% PFA for 12 min
Abdominal aorta opened in the longitudinal direction

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