Flow Cytometric Analysis of Liver Cells

The flow cytometry was carried out using the BD Cell Analyzer (BD Bioscience), and the data were analyzed using FlowJo 10 software (BD Bioscience). The analysis of Kupffer cells and LNPCs was performed as described previously with some modifications [6 (link)]. Briefly, cells were collected and incubated with primary antibodies that might include IgG2a-PE, IgG1-APC, APC anti-human CD11b antibody (Biolegend, #301310), and/or F4/80-PE (eBioscience, #12-4801-82) at 4°C for 30 minutes. Cells were then rinsed with PBS three times followed by flow cytometry analysis.

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Li Y., Wu C., Lee J., Ning Q., Lim J., Eoh H., Wang S., Hurrell B.P., Akbari O, & Ou J.H. (2024). Hepatitis B virus e antigen induces atypical metabolism and differentially regulates programmed cell deaths of macrophages. PLOS Pathogens, 20(3), e1012079.

Publication 2024

BD Cell Analyzer IgG2a-PE IgG1-APC APC anti-human CD11b antibody F4/80-PE

Corresponding Organization : City Of Hope National Medical Center

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Variable analysis

independent variables

Primary antibodies used (IgG2a-PE, IgG1-APC, APC anti-human CD11b antibody, F4/80-PE)

dependent variables

Kupffer cells and LNPCs analyzed

control variables

Cells were incubated with primary antibodies at 4°C for 30 minutes
Cells were rinsed with PBS three times before flow cytometry analysis

controls

No positive or negative controls were explicitly mentioned in the input protocol.

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