Western Blot Analysis of Testicular Proteins

Western blot experiments on whole testes were performed as described in Comptour et al. (2014) (link) and Moretti et al. (2017) (link). Fifteen microliters of immunoprecipitated samples and 5 µl of input were denatured in 1× NuPAGE LDS sample buffer (Life Technologies) at 95 °C during 10 min. Antibody against SLY (Reynard et al. 2009 (link)) and against SLX/SLXL1 (Reynard et al. 2007 (link)) was diluted 1/3,000; anti-TUBULIN was diluted 1/5,000 (05-661, Millipore); and anti-TBLX1R1 (ab24550, Abcam), anti-HDAC3 (ab7030, Abcam), anti-TBL1X (ab24548, Abcam), anti-SSTY1 (Comptour et al. 2014 (link)), anti-SPIN1 (orb193004, Biorbyt), and anti-NCor1 (ab3482, Abcam) were diluted 1/1,000.