Isolation and Culture of HF-MSCs

This study was approved by Ethnic Committee of School of Public Health, Jilin University (2021-06-06). The isolation and identification of HF-MSCs were performed in our previous study (Jiang et al., 2019 (link); Liu et al., 2019 (link); Shi et al., 2019 (link)). HF-MSCs were cultured in Dulbecco’s modified Eagle’s medium (Life Technologies, United States ) containing 10% fetal bovine serum (FBS, Hyclone, United States ), 2 ng/ml basic fibroblast growth factor (Sino Biological Inc., China) and 100 U/ml penicillin-streptomycin (Hyclone, Logan, UT, United States ). HEK293T cells were cultured in DMEM containing 10% FBS and 100 U/ml penicillin-streptomycin. The cells were cultured at 37°C and 5% CO₂. When HF-MSCs or HEK293T proliferated to 80% confluence, they were digested with 0.25% trypsin and subcultured under the same individual conditions.