Synthesis of SN38-Glucose Conjugates

SN38-glucose conjugates were synthesized according to Scheme 1. β-D-Glucopyranosyl azide, 2-azidoethyl β-D-glucopyranoside, and 2-(2-azidoethoxy)ethyl β-D-glucopyranoside were prepared as previously reported (Pintal et al., 2015 (link); Shiao et al., 2016 (link); Palmioli et al., 2017 (link)). ¹H NMR and ¹³C NMR spectra were measured on a Bruker AM400 NMR spectrometer. Proton chemical shifts of NMR spectra were given in ppm relative to internals reference TMS (0.00ppm). High-resolution mass spectra were recorded on a Bruker MicroTOF spectrometer using positive (ESI⁺) or negative electrospray ionization (ESI⁻). HPLC analysis was measured on Waters 2695e equipped with a 2998 PDA detector.