Droplet Digital PCR Experimental Setup

Digital PCR experiments were carried out using the QX200™ Droplet Digital™ PCR System (Bio-Rad Laboratories) following the protocols described earlier [6 (link), 16 (link), 17 ]. A methodical overview of all experimental setups is presented in Supplementary Data 1. Context sequences, PCR annealing temperatures and supplier information for all assays used are provided in Supplementary Table 1.
Raw digital PCR results were acquired using QuantaSoft (version 1.7.4, Bio-Rad Laboratories) and imported in the online digital PCR management and analysis application Roodcom WebAnalysis (version 1.9.4, available via https://webanalysis.roodcom.nl).

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Nell R.J., Menger N.V., Versluis M., Luyten G.P., Verdijk R.M., Madigan M.C., Jager M.J, & van der Velden P.A. (2021). Involvement of mutant and wild-type CYSLTR2 in the development and progression of uveal nevi and melanoma. BMC Cancer, 21, 164.

Publication 2021

QX200™ Droplet Digital™ PCR System QuantaSoft

Assays Digital 1

Corresponding Organization :

Other organizations : Leiden University Medical Center, University of Sydney

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Variable analysis

independent variables

Primer and probe assays

dependent variables

Digital PCR results

control variables

Experimental setup described in Supplementary Data 1
PCR annealing temperatures and supplier information provided in Supplementary Table 1

controls

Positive controls: Not explicitly mentioned
Negative controls: Not explicitly mentioned

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