Visualizing PlMYB108 Transcription Factor

This analysis was done using tobacco leaves and confocal laser microscopy (Nikon C2-ER, Tokyo, Japan). The p35S:PlMYB108-GFP gene fusions were synthesized to retrieve the PlMYB108 product (Figure S1). The ORF of PlMYB108 was amplified using primers carrying BsmB I restriction sites (forward 5′-CAGTGGTCTCACAACATGGATGTTAATGGGAGAGG-3′, reverse 5′-CAGTGGTCTCATACAAATATTGCTGGAGAACTGTT-3′), which were then digested and introduced to the expression vectors with T4 DNA ligase (TaKaRa, Kyoto, Japan) to produce a set of p35S:PlMYB108-GFP fusions, which were then sequenced for further validation. Subsequent transformations of the p35S:PlMYB108-GFP and the empty p35S:GFP vector were conducted as reported previously [32 (link)].