RNA Extraction and qPCR Analysis

Total RNA was extracted by using an RNA extraction kit (Bioflux, Beijing, China) and then subjected to reverse transcription by using Primescript RT Master Mix Kit (Takara, Dalian, China) to synthesize cDNA. Real-time PCR was then performed using a Light Cycler (Roche) with a SYBR Premix Ex Taq II Kit (Takara, Dalian, China). Results were analyzed as described in the previous study (24 (link)). The primers used for qPCR was described in Table 2.

Free full text: Click here

Chen R., Wang Y., Xu Y., He Y., Li Q., Xia C, & Zhang B. (2022). RRBP1 depletion of bone metastatic cancer cells contributes to enhanced expression of the osteoblastic phenotype. Frontiers in Oncology, 12, 1005152.

Publication 2022

RNA extraction kit Primescript RT Master Mix Kit Light Cycler SYBR Premix Ex Taq II Kit

Cdna Light Primers Real time pcr Reverse transcription

Corresponding Organization : Zhongshan Hospital of Xiamen University

Top 5 similar protocols

Variable analysis

independent variables

RNA extraction kit (Bioflux, Beijing, China)
Primescript RT Master Mix Kit (Takara, Dalian, China)
SYBR Premix Ex Taq II Kit (Takara, Dalian, China)

dependent variables

Gene expression measured by real-time PCR

control variables

RNA extraction method
Reverse transcription kit
Real-time PCR kit

controls

Positive control: Not specified
Negative control: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!