Culturing Primary Glioblastoma Cell Lines

GBM cancer stem cell line 0308 was provided by Howard Fine (Department of Neurology, Weill Cornell Medicine, New York, New York, USA) (37 (link), 77 (link)). TS667 from a patient with primary GBM was derived in-house (78 (link)), as was MGG8 from a patient with primary GBM (79 (link)). 0308 and TS667 cells were cultured in Neurobasal media (Life Technologies) supplemented with 0.5× B27 without vitamin A (Thermo Fisher), 0.5× N2 supplement (Thermo Fischer), 2 mM L-glutamine (Thermo Fisher), 1 mM sodium pyruvate (Thermo Fisher), 50 μg/ml EGF (Peprotec), and 50 μg/ml basic FGF (Peprotec). MGG8 cells were cultured in Neurobasal media (Life Technologies) supplemented with 1× B27 without vitamin A, 1× N2 supplement, 3 mM GlutaMAX (Gibco), 5 mg/ml heparin (Stem Cell Technologies), 20 ng/ml EGF (Peprotec), and 20 μg/ml basic FGF (Peprotec).

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Galdieri L., Jash A., Malkova O., Mao D.D., DeSouza P., Chu Y.E., Salter A., Campian J.L., Naegle K.M., Brennan C.W., Wakimoto H., Oh S.T., Kim A.H, & Chheda M.G. (2021). Defining phenotypic and functional heterogeneity of glioblastoma stem cells by mass cytometry. JCI Insight, 6(4), e128456.

Publication 2021

Neurobasal media B27 without vitamin A N2 supplement L-glutamine sodium pyruvate GlutaMAX heparin

Cancer stem cell Cells Cultured media Glutamine Heparin Medicine Patient Pyruvate Sodium Stem cell Supplement Vitamin a

Corresponding Organization : Washington University in St. Louis

Other organizations : Memorial Sloan Kettering Cancer Center, Massachusetts General Hospital

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Variable analysis

independent variables

Cell lines: 0308, TS667, MGG8

dependent variables

Not explicitly mentioned

control variables

Cell culture media and supplements: Neurobasal media, B27 without vitamin A, N2 supplement, L-glutamine, sodium pyruvate, EGF, basic FGF, heparin, GlutaMAX

controls

No positive or negative controls were explicitly mentioned in the input text.

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