Colorectal Cancer Xenograft Mouse Model

The mice were repeatedly treated and weighed every 2 days, and the tumor volume was measured with a vernier caliper according to the equation: volume (mm³) = (length × width²)/2. On day 14, all mice were euthanized, and an autopsy was conducted. The tumors were detached, weighed, and fixed with 10% neutral formalin. Tumor tissues were embedded with paraffin, sectioned at a thickness of 5 μm, and mounted onto slides for histological examination. Tumor growth status and organ tissue lesions were observed using HE staining. Ki-67 assay was used to detect the proliferation of colorectal cancer cells in tumor tissues. The cleaved caspase-3 was investigated by immunohistochemical analysis according to a previously reported method [39 (link)]. The expression of MDR protein ABCB1, ABCC1, and ABCG2 were investigated by immunohistochemical analysis according to a previously reported method.

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Pei M., Liu K., Qu X., Wang K., Chen Q., Zhang Y., Wang X., Wang Z., Li X., Chen F., Qin H, & Zhang Y. (2023). Enzyme-catalyzed synthesis of selenium-doped manganese phosphate for synergistic therapy of drug-resistant colorectal cancer. Journal of Nanobiotechnology, 21, 72.

Publication 2023

Abcb1 protein Abcc1 Assay Autopsy Caspase 3 Cells proliferation Colorectal cancer Embedded paraffin Formalin Mice Tissues Tumor

Corresponding Organization :

Other organizations : Anhui University of Science and Technology, Shanghai Tenth People's Hospital, Tongji University

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Variable analysis

independent variables

Repeated treatment of the mice

dependent variables

Tumor volume (mm^3) calculated using the equation: (length × width^2)/2
Tumor weight
Tumor growth status and organ tissue lesions observed using HE staining
Proliferation of colorectal cancer cells in tumor tissues detected by Ki-67 assay
Expression of MDR proteins ABCB1, ABCC1, and ABCG2 investigated by immunohistochemical analysis

control variables

Weighing the mice every 2 days
Euthanizing all mice on day 14
Detaching, weighing, and fixing the tumors with 10% neutral formalin
Embedding tumor tissues with paraffin, sectioning at 5 μm thickness, and mounting onto slides for histological examination

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