HAP1 cells have been described previously43 (link). A375, A431, A549, DLD1, JY, Raji, RKO and SKBR3 cells were purchased from American Type Culture Collection (ATCC). B16F10 cells were kindly provided by D. Peeper. Ba/F3 cells are bone-marrow-derived, immortalized cells which are IL-3-dependent, as described in44 (link). NKIRTIL006 cell line was generated from a patient treated at the Netherlands Cancer Institute.
HAP1 cells were cultured in IMDM (ThermoFisher Scientific) supplemented with 10% Fetal Calf Serum (FCS, Sigma), 100 U/mL penicillin (Roche) and 100 μg/mL streptomycin (Roche) (penicillin/streptomycin) and L-glutamine. NKIRTIL006 cells were cultured in IMDM supplemented with 10% FCS and penicillin/streptomycin. A375, A549 and B16F10 were cultured in DMEM supplemented with 10% FCS and penicillin/streptomycin. A431, DLD1, Raji and JY cells were cultured in RPMI supplemented with 10% FCS and penicillin/streptomycin. Ba/F3-Her2 cells were cultured in RPMI supplemented with 10% FCS, penicillin/streptomycin, 0.2 ng/mL mouse IL-3 (Immunotools) and 5.0 μg/mL puromycin. SKBR3 cells were cultured in IMDM supplemented with 20% FCS and 100 U/mL penicillin/streptomycin. Cells were cultured at 37°C and 5% CO2.
HAP1 cells were cultured in IMDM (ThermoFisher Scientific) supplemented with 10% Fetal Calf Serum (FCS, Sigma), 100 U/mL penicillin (Roche) and 100 μg/mL streptomycin (Roche) (penicillin/streptomycin) and L-glutamine. NKIRTIL006 cells were cultured in IMDM supplemented with 10% FCS and penicillin/streptomycin. A375, A549 and B16F10 were cultured in DMEM supplemented with 10% FCS and penicillin/streptomycin. A431, DLD1, Raji and JY cells were cultured in RPMI supplemented with 10% FCS and penicillin/streptomycin. Ba/F3-Her2 cells were cultured in RPMI supplemented with 10% FCS, penicillin/streptomycin, 0.2 ng/mL mouse IL-3 (Immunotools) and 5.0 μg/mL puromycin. SKBR3 cells were cultured in IMDM supplemented with 20% FCS and 100 U/mL penicillin/streptomycin. Cells were cultured at 37°C and 5% CO2.
