MeJA-Induced GFP and GUS Expression

Our previously established transgenic chrysanthemum (Chrysanthemum × morifolium) harboring TcCHS-promoter-driven GFP and tobacco (Nicotiana tabacum) harboring TcGLIP-promoter-driven GUS were treated with MeJA (Sultana et al., 2015 (link)). The plants were sprayed with 5 ml of 300 μM MeJA dissolved in 0.8% ethanol as a single foliar application. Leaves were collected in triplicate at 0 (control) and 12 h after treatment, and were immediately frozen in liquid nitrogen. The GFP expression level was determined by quantitative real-time PCR (qRT-PCR) analysis. The GUS activity was measured as previously described (Luo et al., 2013 (link)).