Every morning for four weeks, the animals received fresh dandelion root in a 250 ml volume bottle [6 (link)]. In order to accurately record the intake of dandelion root, each animal was placed in a separate cage, while the volume of tea was recorded daily. The average daily dandelion root intake was 39.44 ± 2.67 ml in the experimental group, while the control group took tap water in an average amount of 42.85 ± 3.16 ml.
The animals were subjected to anesthesia at the end of the experimental protocol prior to sacrifice. A mixture of ketamine (Vet-Agro, Lublin, Poland) and xylazine (De Adelaar B.V, Venray, Holland) was prepared in a syringe. Administration of 25 µl/kg ketamine and 62.5 µl/kg xylazine was equivalent to the recommended dosage of 10 mg ketamine/kg and 5 mg xylazine/kg for rats [11 (link)]. The ketamine/xylazine mixture was administered i.p., and after 2 min, animals were sacrificed by decapitation.
The animals were subjected to anesthesia at the end of the experimental protocol prior to sacrifice. A mixture of ketamine (Vet-Agro, Lublin, Poland) and xylazine (De Adelaar B.V, Venray, Holland) was prepared in a syringe. Administration of 25 µl/kg ketamine and 62.5 µl/kg xylazine was equivalent to the recommended dosage of 10 mg ketamine/kg and 5 mg xylazine/kg for rats [11 (link)]. The ketamine/xylazine mixture was administered i.p., and after 2 min, animals were sacrificed by decapitation.
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