Flies were maintained at 25 °C, 60% relative humidity, and 12-h light/dark cycle. Adults and larvae were reared on a standard cornmeal and yeast-based diet, unless otherwise noted. The Gal4/UAS driven RNAi crosses were cultured at 25°C until eclosion, and then incubated at 29°C for 7 days, and the same culture conditions were also used for the control group.
promE-Gal4 and promE-GS-Gal4 (oeno-specific GAL4) were obtained from Dr. H. Bai(92 ). UAS-fruMA and UAS-fruCOMB were from Dr. M. Arbeitman. elav-Gal4 (BDSC#6920), UAS-fruRNAi (BDSC#31593), UAS-GFP (BDSC#5413), fru-NP21-Gal4 (BDSC# 30027), fru-P1-Gal4 (BDSC#66696), Hnf4::GFP.FLAG (BDSC#38649), UAS-Hnf4RNAi (BDSC#29375), UAS-Hnf4RNAi (BDSC#64988), UAS-dsxRNAi (BDSC#55646), Act5C-GAL4 (BDSC#4414) and W1118 (BDSC#5905) were obtained from the Bloomington Drosophila Stock Center. UAS-fru-gRNA (VDRC#342548), UAS-fruRNAi (VDRC#330035), UAS-fruRNAi (VDRC#105005) were obtained from the Vienna Drosophila Resource Center. UAS-Hnf4::HA (F000144) was obtained from FlyORF (Zurich ORFeome Project).
RU486 (mifepristone, Fisher Scientific) was dissolved in 95% ethanol, and added to standard food at a final concentration of 100 μM for all the experiments. For activation of the GeneSwitch (GS) Gal4 driver, flies were fed on RU486 food for 7 consecutive days, unless otherwise noted.
To temporally induce fru MAGIC clones, early 3rd instar larvae of w; UAS-frug-RNA/Act5C-Gal4; HS-Cas9/+ were heat shocked for 30min at 37°C, and examined at day-7 after eclosion. The HS-Cas9 stock was kindly provided by Dr. C. Han(42 (link)).
promE-Gal4 and promE-GS-Gal4 (oeno-specific GAL4) were obtained from Dr. H. Bai(92 ). UAS-fruMA and UAS-fruCOMB were from Dr. M. Arbeitman. elav-Gal4 (BDSC#6920), UAS-fruRNAi (BDSC#31593), UAS-GFP (BDSC#5413), fru-NP21-Gal4 (BDSC# 30027), fru-P1-Gal4 (BDSC#66696), Hnf4::GFP.FLAG (BDSC#38649), UAS-Hnf4RNAi (BDSC#29375), UAS-Hnf4RNAi (BDSC#64988), UAS-dsxRNAi (BDSC#55646), Act5C-GAL4 (BDSC#4414) and W1118 (BDSC#5905) were obtained from the Bloomington Drosophila Stock Center. UAS-fru-gRNA (VDRC#342548), UAS-fruRNAi (VDRC#330035), UAS-fruRNAi (VDRC#105005) were obtained from the Vienna Drosophila Resource Center. UAS-Hnf4::HA (F000144) was obtained from FlyORF (Zurich ORFeome Project).
RU486 (mifepristone, Fisher Scientific) was dissolved in 95% ethanol, and added to standard food at a final concentration of 100 μM for all the experiments. For activation of the GeneSwitch (GS) Gal4 driver, flies were fed on RU486 food for 7 consecutive days, unless otherwise noted.
To temporally induce fru MAGIC clones, early 3rd instar larvae of w; UAS-frug-RNA/Act5C-Gal4; HS-Cas9/+ were heat shocked for 30min at 37°C, and examined at day-7 after eclosion. The HS-Cas9 stock was kindly provided by Dr. C. Han(42 (link)).
