Protocol detail

Immunohistochemical Staining of Mouse Lung

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Formalin-fixed paraffin-embedded mouse lung tissue was used for staining with AOX antiserum (1:2000; custom raised in rabbit; 21st Century Biochemicals, Marlborough, MA, USA) (42 (link)), visualized using the ZytoChem Plus (HRP) Polymer Bulk Kit (Zytomed Systems GmbH, Berlin, Germany) and VECTOR NovaRED Peroxidase (HRP) Substrate Kit (Vector Laboratories, Burlingame, USA).

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Sommer N., Alebrahimdehkordi N., Pak O., Knoepp F., Strielkov I., Scheibe S., Dufour E., Andjelković A., Sydykov A., Saraji A., Petrovic A., Quanz K., Hecker M., Kumar M., Wahl J., Kraut S., Seeger W., Schermuly R.T., Ghofrani H.A., Ramser K., Braun T., Jacobs H.T., Weissmann N, & Szibor M. (2020). Bypassing mitochondrial complex III using alternative oxidase inhibits acute pulmonary oxygen sensing. Science Advances, 6(16), eaba0694.

Publication 2020

VECTOR NovaRED Peroxidase (HRP) Substrate Kit

Antiserum Bulk Formalin Lung Mouse Paraffin Peroxidase Polymer Rabbit Tissue Vector

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Variable analysis

independent variables

Formalin-fixed paraffin-embedded mouse lung tissue

dependent variables

Staining with AOX antiserum

control variables

AOX antiserum (1:2000; custom raised in rabbit; 21st Century Biochemicals, Marlborough, MA, USA)

controls

None specified
None specified

Annotations

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