Modulation of EMT by IL-6 in Ovarian Cancer
Corresponding Organization : Logistics University of People's Armed Police Force
Other organizations : JiangSu Armed Police General Hospital, Chinese PLA General Hospital, Chinese People's Liberation Army, Tibet Autonomous Region People's Hospital
Variable analysis
- Recombinant human IL-6 (Peprotech, Rocky Hill, NJ, USA)
- Pharmacological inhibitors of STAT3 (AG490)
- Pharmacological inhibitors of HIF-1α (YC-1)
- EMT phenotype of A2780 and SKOV3 cells
- A2780 cells were cultured in the presence of IL-6 (50 ng/ml) for 24 hours
- SKOV3 cells were cultured in the presence of IL-6 (10 ng/ml) for 48 hours
- A2780 and SKOV-3 cells were treated with YC-1 (10 μM) for 1 hour prior to the addition of exogenous IL-6
- Cells were pretreated with AG490 (50 μM) 30 minutes prior to the addition of IL-6
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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