Peritoneal Surface Sampling Protocol

During the CS realisation each cow was sampled twice. The samples were collected just after replacing the sutured uterus in the abdominal cavity before the closure of the abdominal wall. At this moment, swabs (STERILER^®, Piove di Sacco, Italy) were taken by swiping a long line of 10 cm, 2 cm in parallel to the uterus suture (visceral peritoneum of the uterus) and a 10 cm long line perpendicular to and below the abdominal wall incision (parietal peritoneum).

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Djebala S., Coria E., Munaut F., Gille L., Eppe J., Moula N., Taminiau B., Daube G, & Bossaert P. (2023). Bacterial Contamination of the Surgical Site at the Time of Elective Caesarean Section in Belgian Blue Cows—Part 2: Identified by 16Sr DNA Amplicon Sequencing. Veterinary Sciences, 10(2), 94.

Publication 2023

Abdominal cavity Abdominal wall Parietal peritoneum Suture Uterus Visceral peritoneum

Corresponding Organization : University of Liège

Other organizations : University of Namur

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Variable analysis

independent variables

Location of sample collection (visceral peritoneum of the uterus, parietal peritoneum of the abdominal wall)

dependent variables

Presence and/or abundance of microorganisms (not explicitly mentioned, but can be inferred from the sample collection)

control variables

Time of sample collection (just after replacing the sutured uterus in the abdominal cavity before the closure of the abdominal wall)
Number of samples collected per cow (two samples per cow)

controls

No positive or negative controls were explicitly mentioned in the provided information.

Annotations

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