The temperature, pH, dissolved oxygen (DO), and salinity were measured daily at 1100~1300 h with a handheld multi-parameter meter (YSI Professional Plus, YSI, Yellow Springs, OH, USA) during the entire experiment, same procedures as in [25 (link)].
The dissolved inorganic nitrogen (NH3-N, NO2-N, and NO3-N), phosphorus (PO4-P), and total Fe concentrations of water samples that had been filtered through 0.45 μm filter paper were analyzed daily for each tank. We used HACH water analysis products (HACH, Loveland, CO, USA) such as the ammonia kit (salicylate method 8155), nitrite kit (diazotization method 8507), phosphorus kit (ascorbic acid method 8048), and total iron kit (FerroVer® method 8008) in the analyses, and measured the nutrient concentrations with a spectrophotometer (Synergy H4 Hybrid Reader, BioTek Instruments, Winooski, VT, USA) [25 (link)]. Nitrate (NO3−) was reduced to NO2− [60 (link)] before using the HACH nitrite kit.
The dissolved inorganic nitrogen (NH3-N, NO2-N, and NO3-N), phosphorus (PO4-P), and total Fe concentrations of water samples that had been filtered through 0.45 μm filter paper were analyzed daily for each tank. We used HACH water analysis products (HACH, Loveland, CO, USA) such as the ammonia kit (salicylate method 8155), nitrite kit (diazotization method 8507), phosphorus kit (ascorbic acid method 8048), and total iron kit (FerroVer® method 8008) in the analyses, and measured the nutrient concentrations with a spectrophotometer (Synergy H4 Hybrid Reader, BioTek Instruments, Winooski, VT, USA) [25 (link)]. Nitrate (NO3−) was reduced to NO2− [60 (link)] before using the HACH nitrite kit.
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