The A. platensis powder was purchased from a local supplier in Shanghai. Strains used in this study were isolated from the sediment of the South China Sea and stored in the lab: Pseudoalteromonas sp. JS4-1 (GenBank accession number: MT116988), Pseudoalteromonas sp. B27-3, Pseudoalteromonas sp. B47-6, Pseudoalteromonas sp. B62-3, Pseudoalteromonas sp. WH05-1, Pseudoalteromonas sp. WH06-2, and Pseudoalteromonas sp. ZB23-2. Immortal human umbilical vein endothelial cell line (HUVECs) and human immortal keratinocyte line (HaCaT) were obtained from the National Collection of Authenticated Cell Cultures (Shanghai, China). Wild type C. elegans (N2) were obtained from Caenorhabditis Genetics Center (CGC).
Ninhydrin, penicillin-streptomycin solution (cell culture grade), trypsin-EDTA solution, dimethyl sulfoxide (DMSO), thiazolyl blue tetrazolium bromide (MTT), and phosphate buffered saline (PBS) were purchased from Sangon Biotech (Shanghai, China). Trisodium citrate dihydrate, SnCl2, n-propanol, FeSO4, 30% H2O2, cholesterol and glucose were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). In addition, 1,1-diphenyl-2-picrylhydrazyl (DPPH), 1,10-phenanthroline monohydrate (OP), fluorescein sodium salt, 2,20-azobis (2-amidinopropane) dihydrochloride (AAPH), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), L-leucine, antifade mounting medium and NaN3 were purchased from Sigma-Aldrich, Ltd. (St. Louis, MO, USA). AmiconTM Ultra-15 centrifugal filter units were purchased from Millipore® (Billerica, MA, USA). Sephadex LH-20 was purchased from GE Healthcare Life Sciences (Uppsala, Sweden). Fetal bovine serum (FBS) was purchased from PAN-BiotechGibco company (Aidenbach, Germany). RPMI-1640 medium was purchased from Gibco® ThermoFisher Scientific Company (Waltham, MA, USA). Methyl viologen dichloride was purchased from Aladdin® (Shanghai, China). BCA assay kit, cell lysis buffer, ROS assay kit, catalase (CAT) assay kit, total superoxide dismutase (SOD) assay kit with NBT, cellular glutathione peroxidase (GSH-Px) assay kit with NADPH and lipid peroxidation MDA assay kit were purchased from Beyotime Biotechnology (Shanghai, China).
Ninhydrin, penicillin-streptomycin solution (cell culture grade), trypsin-EDTA solution, dimethyl sulfoxide (DMSO), thiazolyl blue tetrazolium bromide (MTT), and phosphate buffered saline (PBS) were purchased from Sangon Biotech (Shanghai, China). Trisodium citrate dihydrate, SnCl2, n-propanol, FeSO4, 30% H2O2, cholesterol and glucose were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). In addition, 1,1-diphenyl-2-picrylhydrazyl (DPPH), 1,10-phenanthroline monohydrate (OP), fluorescein sodium salt, 2,20-azobis (2-amidinopropane) dihydrochloride (AAPH), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), L-leucine, antifade mounting medium and NaN3 were purchased from Sigma-Aldrich, Ltd. (St. Louis, MO, USA). AmiconTM Ultra-15 centrifugal filter units were purchased from Millipore® (Billerica, MA, USA). Sephadex LH-20 was purchased from GE Healthcare Life Sciences (Uppsala, Sweden). Fetal bovine serum (FBS) was purchased from PAN-BiotechGibco company (Aidenbach, Germany). RPMI-1640 medium was purchased from Gibco® ThermoFisher Scientific Company (Waltham, MA, USA). Methyl viologen dichloride was purchased from Aladdin® (Shanghai, China). BCA assay kit, cell lysis buffer, ROS assay kit, catalase (CAT) assay kit, total superoxide dismutase (SOD) assay kit with NBT, cellular glutathione peroxidase (GSH-Px) assay kit with NADPH and lipid peroxidation MDA assay kit were purchased from Beyotime Biotechnology (Shanghai, China).
Free full text: Click here
